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The second response cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water link as products (Fig. 9) - UV/Vis. As attracted Fig - https://pxhere.com/en/photographer/4182440. 9, the reaction may also include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As shown in Fig. 9, this might be because the H4B extreme function as an electron sink in a later point in the reaction, so regarding permit NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.